Journal: medRxiv
Article Title: Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy
doi: 10.1101/2021.03.25.21254204
Figure Lengend Snippet: (a) CPLX1 expression levels of mRNA on 14 gastric cancer (GC) cell lines and FHs74, nontumorigenic tubular epithelial cell line. (b) CPLX1-knockdown effect on three GC cell lines at mRNA levels. (c) Proliferation assay comparing those four GC cell lines between untreated, transfected by siControl and siCPLX1. Apoptotic cell observation (d) at cultured on dishes through a phase-contrast microscope, and (e) by staining with annexin-V. Those four GC cell lines were compared between untreated, treated by siControl, treated by siCPLX1. Cells treated by UV-irradiation were positive controls. (f) Migration assay and (g) invasion assay on AGS cells. *P < 0.05, **P < 0.01, ***P < 0.001.
Article Snippet: Gastric cancer cell lines (AGS, RRID is CVCL_0139; GCIY, CVCL_1228; IM95, CVCL_2961; KATOIII, CVCL_0371; MKN1, CVCL_1415; MKN7, CVCL_1417; MKN45, CVCL_0434; MKN74, CVCL_2791; N87, CVCL_1603; NUGC2, CVCL_1611; NUGC3, CVCL_1612; NUGC4, CVCL_3082; OCUM1, CVCL_3084; and SC-6-JCK, CVCL_F953) and the non-tumorigenic tubular epithelial cell line FHs74 (CVCL_2899) were obtained from the American Type Culture Collection (Manassas, VA, USA), the Japanese Collection of Research Bio Resources Cell Bank (Osaka, Japan), or were established at our institute.
Techniques: Expressing, Knockdown, Proliferation Assay, Transfection, Cell Culture, Microscopy, Staining, Irradiation, Migration, Invasion Assay
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